Part:BBa_K4179000:Design
Petroselinum crispum prenyl transferase (PcPT)
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 127
Illegal EcoRI site found at 559
Illegal EcoRI site found at 922
Illegal PstI site found at 82
Illegal PstI site found at 445 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 127
Illegal EcoRI site found at 559
Illegal EcoRI site found at 922
Illegal NheI site found at 1207
Illegal PstI site found at 82
Illegal PstI site found at 445 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 127
Illegal EcoRI site found at 559
Illegal EcoRI site found at 922 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 127
Illegal EcoRI site found at 559
Illegal EcoRI site found at 922
Illegal PstI site found at 82
Illegal PstI site found at 445 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 127
Illegal EcoRI site found at 559
Illegal EcoRI site found at 922
Illegal PstI site found at 82
Illegal PstI site found at 445 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
• This gene was codon optimized for E. coli.
• In order to offer this part to all iGEM teams to use, a SapI restriction site had to be eliminated, making the sequence iGEM Type IIS RFC [1000] compatible. This can be done by adding a silent mutation to any bp in the range 1034-1040. When adding this part’s sequence to the registry the team made a silent mutation at the 1038 location, substituting T with A.
Source
PcPT gene was identified in Petroselinum crispum (parsley) [1], however the sequence in this part’s registry page is taken from #172654 plasmid from AddGene, which was codon optimized for E. coli [2].
References
1. Karamat F, Olry A, Munakata R et al. A coumarin-specific prenyltransferase catalyzes the crucial biosynthetic reaction for furanocoumarin formation in parsley. The Plant Journal 2014; 77: 627–638.
2. Bu X-L, He B-B, Weng J-Y et al. Constructing Microbial Hosts for the Production of Benzoheterocyclic Derivatives. ACS Synth Biol 2020; 9: 2282–2290.